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exon array
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Gene level analysis of Affymetrix GeneChip Mouse Exon Arrays
affy
aroma.affymetrix
exon array
xps
oligo
7.4 years ago
Gordon Smyth
52k
2
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MAS5 algorithm in mas5() function of xps function when process Human Gene 1.0 ST Array or Human Exon 1.0 ST Array
xps
MAS5 algorithm
Human Gene 1.0 ST Array
Exon Array
8.3 years ago
linweili_tj
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Comment: What type of counts data to import for performing Isoform analysis in edgeR
by
mohammedtoufiq91
▴ 10
@gordonsmyth I am exploring workflows for differential transcript expression analysis and have been using the nfcore rnasplice pipeline, w…
Answer: What type of counts data to import for performing Isoform analysis in edgeR
by
Gordon Smyth
52k
I haven't used nf-core but, from browsing through their documentation, they didn't seem to have any pipeline for differential transcript ex…
Comment: What type of counts data to import for performing Isoform analysis in edgeR
by
Gordon Smyth
52k
The poster needs to run Salmon with `--numGibbsSamples=50`. I suspect that nf-core just won't do that, so Salmon needs to be run directly.
Comment: What type of counts data to import for performing Isoform analysis in edgeR
by
ATpoint
★ 4.6k
Probably nf-core did not run with bootstrap/gibbs replicates. Check the code. See salmon docs on how to turn that on.
Comment: What type of counts data to import for performing Isoform analysis in edgeR
by
mohammedtoufiq91
▴ 10
@atpoint Thank you. I did wanted to try using the `quant.sf` files from the `salmon` run by referring edgeR user guide example `4.6 Differe…
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Answer: What type of counts data to import for performing Isoform analysis in edgeR
Transcript-level differential expression using DESeq2
Comment: What type of counts data to import for performing Isoform analysis in edgeR
Answer: What type of counts data to import for performing Isoform analysis in edgeR
Comment: ANOVA like approach of edgeR
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